Development of STS Marker KC1 for Brown Planthopper Resistance Gene Bph18(t) and Marker Assisted  Selection Efficiency

doi:10.3969/j.issn.1001-7216.2010.03.006

Chinese Journal of Rice Science

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Development of STS Marker KC1 for Brown Planthopper Resistance Gene Bph18(t) and Marker Assisted Selection Efficiency

LIANG Yun-tao^1，2，4,#, WANG Chun-lian^1,#, LAI Feng-xiang³, LIU Pi-qing², WANG Jian¹, FU Qiang³, ZHAO Kai-jun^1,*

¹Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture/The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081， China； ²Agricultural College, Guangxi University, Nanning 530005, China； ³China National Rice Research Institute, Hangzhou 310006, China；⁴ Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China； ^# These authors contributed equally to this paper； *Corresponding author, E-mail： zhaokj@mail.caas.net.cn

Received:1900-01-01 Revised:1900-01-01 Online:2010-05-10 Published:2010-05-10

水稻抗褐飞虱基因Bph18(t)的STS标记开发及有效性验证

梁云涛^1，2，4,#，王春连^1,#，赖凤香³，刘丕庆²，王坚¹，傅强³，赵开军^1,*

¹中国农业科学院作物科学研究所/农业部作物遗传育种重点实验室/国家农作物基因资源与遗传改良重大科学工程，北京 100081； ²广西大学农学院，广西南宁530005; ³中国水稻研究所，浙江杭州 310006； ⁴广西农业科学院水稻研究所，广西南宁 530007； ^#共同第一作者； *通讯联系人， E-mail：zhaokj@mail.caas.net.cn

Abstract

Abstract: To facilitate application of the elite brown planthopper(BPH) resistance gene Bph18(t) to rice breeding program by molecular assisted selection(MAS), a STS marker, KC1, for Bph18(t) gene was developed. To test the MAS efficiency of KC1 for Bph18(t), the F2 plants derived from the cross between Yangdao 6(i.e.9311)\[without Bph18(t), susceptible to BPH\] and C4064 \[harboring Bph18(t)\] were subjected to both bioassay of BPH resistance in the field and molecular identification with the STS marker KC1 in the laboratory. Among the F2 plants, 292 plants were resistant to BPH while the other 84 plants were susceptible, indicating a single gene \[Bph18(t)\] controlling the BPH resistance in the F2 population. The results of molecular identification showed that the MAS efficiency of KC1 for Bph18(t) was 82.6%, indicating that the STS marker KC1 could be useful to trail Bph18(t) gene in rice breeding program.

Key words: resistance gene, molecular marker, rice brown planthopper, marker assisted selection

摘要： 利用携有Bph18(t)基因抗褐飞虱材料与感虫材料之间在抗虫位点上的单核苷酸差异，成功开发出1个STS标记KC1。该标记可以准确区分含或不含抗虫基因Bph18(t)的基因型。进一步构建扬稻6号（9311）\[不含Bph18(t)基因，感褐飞虱\]/C4064\[携有Bph18(t)基因\]的F2群体进行抗虫鉴定，同时使用KC1标记检测F2群体单株的基因型。根据分子标记检测结果与抗虫表现之间的符合程度推算出标记的选择效果达到了82.6%。研究结果表明，KC1标记有较高的目的基因选择效率，可以应用于扬稻6号遗传背景下Bph18(t) 基因的分子标记辅助选择。

关键词: 抗性基因, 分子标记, 水稻褐飞虱, 分子标记辅助选择

LIANG Yun-tao,#,WANG Chun-lian,#, LAI Feng-xiang, LIU Pi-qing,WANG Jian,FU Qiang,ZHAO Kai-jun. Development of STS Marker KC1 for Brown Planthopper Resistance Gene Bph18(t) and Marker Assisted Selection Efficiency[J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2010.03.006 .

梁云涛,王春连,赖凤香,刘丕庆,王坚,傅强,赵开军,. 水稻抗褐飞虱基因Bph18(t)的STS标记开发及有效性验证
[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2010.03.006 .

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Development of STS Marker KC1 for Brown Planthopper Resistance Gene Bph18(t) and Marker Assisted Selection Efficiency

水稻抗褐飞虱基因Bph18(t)的STS标记开发及有效性验证

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